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cfse staining

Amine-reactive dye CFSE (carboxyfluorescein diacetate, succinimidyl ester, also known as CFDA-SE) is widely used in cell proliferation and in vivo cell tracking. CFSE is non-fluorescent until it enters viable cells, where it is hydrolyzed by cytoplasmic esterase enzymes to release fluorescent amine-reactive dyes. These dyes then...

My 7 year old loves to tell people "bite my shiny metal ass" and it's awesome.

Wife and I decided to do another watch through before the new episodes. The 7 year old caught a couple and now he’s hooked. His favorites to quote are Bender and Zoidberg. It’s especially cute when he goes “wub wub wub!” At the grocery store. I’m so proud.

What are the best instruments for a beginner to pick up?

So i have (i think) no mucical talent becasue in school i was always bad at music classes and was behind compared to others also i coudent stand all the noises going on. im not sure why but i wanted a keyboard pinao becasue i like the way they sound, once i got one i tried to have some folks teach me but i imeditly got...

Career advice: should I give this interviewer a second chance?

Yesterday I had an interview for a certain position. Last Monday it had been scheduled for one location but on Friday it got moved to another. At the last minute yesterday, apparently it had gotten switched to the original interview location, and I was not notified until I got to that second location and waited a few minutes. I...

I like the transition away from prioritizing comments over questions.

On Reddit, my karma was always weighted more on the question side than on the comment side. I felt bad for not being a valuable contributor to people’s lives rather than being selfish and always asking things for myself. Lemmy has gotten rid of that point system so now I feel like I can feel free to ask as many questions as I...

Question regarding partitioning

So, basically I’d like to replace the /home with different hard drives. I have a 4tb one that I’m using for videos, a 1tb I’m using for audio files and video games (not the actual game installed there but for example executables or ROMs), and a 500gb I’m using for texts and images. My idea would be to not have the...

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