Interesting read, I guess testing on mice is all you can do the behavioral part. But the methods section doesn’t state any weaknesses, I know it’s nor required but you know.
At the end of the 3-week-long exposure, behavioral testing began. During the open-field test, mice were allowed to explore a low-lit chamber for 90 min with spontaneous movements monitored in the x-, y-, and z-directions. Several parameters to measure behavioral performance were recorded, including distance traveled, rearing activity, and duration in the center. Surprisingly, we found that acute exposure to PS-MPs induced an increase in distance traveled, which was more pronounced in older animals (Figure 3A–D). Similarly, both young and old PS-MP-exposed mice reared significantly more in the open-field, as compared to age-matched controls (Figure 3E–H). Young PS-MP-exposed mice did not spend more time in the center of the chamber overall (Figure 3J), but both low- and high-dose groups spent more time in the center when analyzed as a function of time (Figure 3I). Low- and medium-dose older animals also showed an increased duration in the center (Figure 3K,L).